{"id":113182,"date":"2025-10-14T10:10:43","date_gmt":"2025-10-14T08:10:43","guid":{"rendered":"http:\/\/wordpress.p697052.webspaceconfig.de\/range-of-services\/next-generation-sequencing\/"},"modified":"2026-03-19T16:53:18","modified_gmt":"2026-03-19T15:53:18","slug":"next-generation-sequencing","status":"publish","type":"page","link":"https:\/\/www.molpath-bw.de\/en\/range-of-services\/next-generation-sequencing\/","title":{"rendered":"Next Generation Sequencing"},"content":{"rendered":"\n
<\/div><\/div><\/div><\/div>
<\/div><\/div><\/div><\/div>
<\/div><\/div><\/div><\/div>
<\/div><\/div><\/div><\/div><\/div><\/div>

Next Generation Sequencing<\/strong><\/h1>\n

<\/h1>\n<\/div><\/div><\/div><\/div><\/div>

We offer the following services:<\/strong><\/p>\n<\/div><\/div>

Oncomine\u2122 Dx Express Test (CE-IVD, Mutations, Amplifications, Fusions)<\/h2>

Gene hotspot regions:<\/strong>
AKT1, AKT2, AKT3, ALK, AR, ARAF, BRAF, CDK4, CHEK2, CTNNB1, EGFR, ERBB2, ERBB3, ERBB4, ESR1, FGFR1, FGFR2, FGFR3, FGFR4, FLT3, GNAS, HRAS, IDH1, IDH2, KEAP1, KIT, KRAS, MAP2K1, MAP2K2, MET, NRAS, NTRK1, NTRK2, NTRK3, PDGFRA, PIK3CA, PTEN, RAF1, RET, ROS1, STK11, TP53<\/em><\/p>\n

Gene amplifications:<\/strong>
AR, EGFR, ERBB2, ERBB3, FGFR1, FGFR2, FGFR3, KRAS, MET, PIK3CA<\/em><\/p>\n

Gene fusions (intergenic and intragenic):<\/strong>
ALK, AR, BRAF, ESR1, FGFR1, FGFR2, FGFR3, MET, NRG1, NTRK1, NTRK2, NTRK3, NUTM1, RET, ROS1, RSPO2, RSPO3<\/em><\/p>\n<\/div><\/div>

Custom-Assay V1 (Mutations)<\/h2>

Gene hotspot regions:<\/strong>
ESR1, POLE<\/em><\/p>\n<\/div><\/div>

Custom-Assay V2 (Mutations)<\/h2>

Gene hotspot regions:<\/strong>
AKT1, BRAF, CTNNB1, EGFR (ohne p.T790M), ERBB2, ESR1, IDH1, IDH2, KRAS, NRAS, PIK3CA, POLE, KIT p.D816V<\/em><\/p>\n

Gene fusions:<\/strong>
NTRK1, NTRK2 und NTRK3<\/em><\/p>\n<\/div><\/div>

OncomineTM Comprehensive Assay Plus (Mutations, Fusions, Copy number variations, TMB, HRD, and MSI analysis)<\/h2>

Gene hotspot regions:<\/strong>
ACVR1, ATP1A1, BCR, BMP5, BTK, CACNA1D, CD79B, CSF1R, CTNNB1, CUL1, CYSLTR2, DGCR8, DROSHA, E2F1, EPAS1, FGF7, FOXL2, FOXO1, GLI1, GNA11, GNAQ, H2BC5, (HIST1H2BD), H3C2, (HIST1H3B), HIF1A, HRAS, IDH1, IL6ST, IRF4, IRS4, KLF4, KNSTRN, MAP2K2, MED12, MYOD1, NSD2, NT5C2, NTRK2, NUP93, PAX5, PIK3CD, PIK3CG, PTPRD, RGS7, RHOA, RPL10, SIX1, SIX2, SNCAIP, SOS1, SOX2, SRSF2, STAT5B, TAF1, TGFBR1, TRRAP, TSHR, WAS<\/em><\/p>\n

Gene amplifications:<\/strong>
ABCB1, CTNND2, DDR1, EMSY, FGF19, FGF23, FGF3, FGF4, FGF9, FYN, GLI3, IGF1R, MCL1, MDM2, MYCL, RPS6KB1, RPTOR, YAP1, YES1<\/em><\/p>\n

Hotspot regions and amplifications of genes:<\/strong>
ABL1, ABL2, AKT1, AKT2, AKT3, ALK, AR, ARAF, AURKA, AURKC, AXL, BCL2, BCL2L12, BCL6, BRAF, CARD11, CBL, CCND1, CCND2, CCND3, CCNE1, CDK4, CDK6, CHD4, DDR2, EGFR, EIF1AX, ERBB2, ERBB3, ERBB4, ESR1, EZH2, FAM135B, FGFR1, FGFR2, FGFR3, FGFR4, FLT3, FLT4, FOXA1, GATA2, GNAS, H3-3A (H3F3A), H3-3B (H3F3B), IDH2, IKBKB, IL7R, KDR, KIT, KLF5, KRAS, MAGOH, MAP2K1, MAPK1, MAX, MDM4, MECOM, MEF2B, MET, MITF, MPL, MTOR, MYC, MYCN, MYD88, NFE2L2, NRAS, NTRK1, NTRK3, PCBP1, PDGFRA, PDGFRB, PIK3C2B, PIK3CA, PIK3CB, PIK3R2, PIM1, PLCG1, PPP2R1A, PPP6C, PRKACA, PTPN11, PXDNL, RAC1, RAF1, RARA, RET, RHEB, RICTOR, RIT1, ROS1, SETBP1, SF3B1, SLCO1B3, SMC1A, SMO, SPOP, SRC, STAT3, STAT6, TERT, TOP1, TPMT, U2AF1, USP8, XPO1, ZNF217, ZNF429<\/em><\/p>\n

Complete coding sequence of the genes:<\/strong>
CALR, CIITA, CYP2D6, ERCC5, FAS, ID3, KLHL13, MTUS2, PSMB10, PSMB8, PSMB9, RNASEH2C, RPL22, RPL5, RUNX1T1, SDHC, SOCS1, STAT1, TMEM132D, UGT1A1, ZBTB20<\/em><\/p>\n

Complete coding sequence of genes and losses at the CNV level:<\/strong>
ABRAXAS1, ACVR1B, ACVR2A, ADAMTS12, ADAMTS2, AMER1, APC, ARHGAP35, ARID1A, ARID1B, ARID2, ARID5B, ASXL1, ASXL2, ATM*, ATR, ATRX, AXIN1, AXIN2, B2M, BAP1, BARD1*, BCOR, BLM,, BMPR2, BRCA1* ,BRCA2*, BRIP1*, CASP8, CBFB, CD274, CD276, CDC73, CDH1, CDH10, CDK12*,CDKN1A, CDKN1B, CDKN2A, CDKN2B, CDKN2C, CHEK1*, CHEK2*, CIC, CREBBP, CSMD3, CTCF, CTLA4, CUL3, CUL4A, CUL4B, CYLD, CYP2C9, DAXX, DDX3X, DICER1, DNMT3A, DOCK3, DPYD, DSC1, DSC3, ELF3, ENO1, EP300, EPCAM, EPHA2, ERAP1, ERAP2, ERCC2, ERCC4, ERRFI1, ETV6, FANCA, FANCC, FANCD2, FANCE, FANCF, FANCG, FANCI, FANCL*, FANCM, FAT1, FBXW7, FUBP1, GATA3, GNA13, GPS2, HDAC2, HDAC9, HLA-A, HLA-B, HNF1A, INPP4B, JAK1, JAK2, JAK3, KDM5C, KDM6A, KEAP1, KMT2A, KMT2B, KMT2C, KMT2D, LARP4B, LATS1, LATS2, MAP2K4, MAP2K7, MAP3K1, MAP3K4, MAPK8, MEN1, MGA, MLH1, MLH3, MRE11, MSH2, MSH3, MSH6, MTAP, MUTYH, NBN*, NCOR1, NF1, NF2, NOTCH1, NOTCH2, NOTCH3, NOTCH4, PALB2*, PARP1, PARP2, PARP3, PARP4, PBRM1, PDCD1, PDCD1LG2, PDIA3, PGD, PHF6, PIK3R1, PMS1, PMS2, POLD1, POLE, POT1, PPM1D, PPP2R2A, PRDM1, PRDM9, PRKAR1A, PTCH1, PTEN, PTPRT, RAD50, RAD51, RAD51B*, RAD51C*, RAD51D*, RAD52, RAD54L*, RASA1, RASA2, RB1, RBM10, RECQL4, RNASEH2A, RNASEH2B, RNF43, RPA1, RUNX1, SDHA, SDHB, SDHD, SETD2, SLX4, SMAD2, SMAD4, SMARCA4, SMARCB1, SOX9, SPEN, STAG2, STK11, SUFU, TAP1, TAP2, TBX3, TCF7L2, TET2, TGFBR2, TNFAIP3, TNFRSF14, TP53, TP63, TPP2, TSC1, TSC2, USP9X, VHL, WT1, XRCC2, XRCC3, ZFHX3, ZMYM3<\/em><\/p>\n

* HRR genes (LOH detection possible at the gene level)<\/p>\n

Gene fusions (intergenic and intragenic):<\/strong>
AKT1, AKT2, AKT3, ALK, AR, BRAF, BRCA1, CDKN2A, EGFR, ERBB2, ERBB4, ERG, ESR1, ETV1, ETV4, ETV5, FGFR1, FGFR2, FGFR3, MAP3K8, MET, MTAP, MYB, MYBL1, NOTCH1, NOTCH2, NOTCH3, NRG1, NTRK1, NTRK2, NTRK3, NUTM1, PIK3CA, PIK3CB, PPARG, PRKACA, PRKACB, RAF1, RARA, RELA, RET, ROS1, RSPO2, RSPO3, STAT6, TERT, TFE3, TFEB, YAP1<\/em><\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":113160,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-113182","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.molpath-bw.de\/en\/wp-json\/wp\/v2\/pages\/113182","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.molpath-bw.de\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.molpath-bw.de\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.molpath-bw.de\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.molpath-bw.de\/en\/wp-json\/wp\/v2\/comments?post=113182"}],"version-history":[{"count":0,"href":"https:\/\/www.molpath-bw.de\/en\/wp-json\/wp\/v2\/pages\/113182\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.molpath-bw.de\/en\/wp-json\/wp\/v2\/pages\/113160"}],"wp:attachment":[{"href":"https:\/\/www.molpath-bw.de\/en\/wp-json\/wp\/v2\/media?parent=113182"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}